The study examines the understanding of the glory of God in the thought of John Calvin. The examination is carried out from the historical observation in the first part and the systematic evaluation in the second part. The author describes the development of the concept of gloria Dei in Calvin’s Institutes as well as its significant role as a counterpart to the major Christian doctrines. Following a survey of the historical background, the presence of gloria Dei in the first, second, and last editions of the Institutes is discussed. In the systematic part, the concept of gloria Dei is analyzed in the context of its dynamic presence throughout the central doctrines such as the doctrine of creation, anthropology, Christology, soteriology, eschatology, and ecclesiology. The systematic evaluation shows that gloria Dei is one of the loudest cantus firmi in Calvin’s theological composition.